Antibiotic Activity in Traditional Medicine

IGN Biosciences

Science Bridge


Antibiotic activity in traditional medicine

The project investigates antibiotic activity in herbal extracts.

 

Age group:

13 to 18 years

Number of participants:

Class up to 20 pupils

Duration:

One day introduction and setting up the experiment with the Science Bridge Team.

Next day evaluation of results and discussion with teacher and Science Bridge Team.

 

Experiment

Extracts are prepared from different plants that are used in Indonesian herbal medicine.

Extracts are applied to filter discs and placed on thin bacterial lawns on petri dishes. Growth inhibition is observed after incubation for 24 hours.

 

Materials

For the introduction, a PowerPoint presentation is prepared to explain the back ground of antibiotics and the problem of antibiotic resistance.

A detailed protocol is established how to prepare extracts, how to prepare bacterial plates and how to prepare the filter discs.

The practical work is accompanied by a PowerPoint presentation showing in detail how the experiment is done.

 

After 24 hours of incubation the plates are examined and inhibition zones are measured in comparison to a conventional antibiotic. Results are documented by photographs.

 

Implementation

Two schools in Malang will be contacted for pilot experiments and the experiment will be carried out with one class each. The experience of these courses will be used to further improve the experiment and to adjust protocols.

 

Perspectives

The experiment can be continued by fractionation of the extract with chromatographic methods.

With the identification of a good antibiotic producer, local variants of this plant may be investigated to determine biodiversity in antibiotic production and to find the most efficient producer.

These further experiments may be carried out by a very interested class or by the Science Bridge Team as a special project.

We may establish a Facebook site to show and discuss the results from the participants and to provide further information.

 

Further measures

The project is currently carried out with the generous support of the Central Laboratory at Brawijaya University.

For future financial support, we have applied for the Anders Award of the German Genetics Society.

We are also preparing for a Crowd Funding initiative to obtain money for basic equipment to establish a Science Bridge laboratory.

 

Dissemination of the project

On this page we will report on further progress in the development of the project.

With successful implementation of the pilot courses we will offer a training course for interested groups of students and lecturers from IGN partner universities.

Teaching materials will be made available to the other IGN partners.

 

Progress as of Dec. 30th, 2015

 

Current team:

 

Name Email Responsibility
Pupimadita Tizar Afdora pupimadita.tizar@gmail.com Laboratory Experiment
Viky Vidayanti merahputihjaya@gmail.com Laboratory Experiment
Isyatul Azizah azizah.4fn@gmail.com Laboratory Experiment
Anggia Noor R. gieule22@gmail.com Laboratory Experiment
Melati Fitriana melatifitriana@ymail.com Laboratory Experiment
Didik Huswo Utomo dknatow@gmail.com Public Relation and Laboratory Experiment
Nadya Veronica nadyavero96@gmail.com Public Relation and Crowd Funding
Nurul Dluha nurul.dluha@gmail.com Child Education
Teaching material
Yustika Aulia yustikaaulia23@gmail.com
Laila Rahmah lailarahmah8@gmail.com Anders Award
Viol Dhea Kharisma violdhea@gmail.com The IT Guys
Syahputra W. putrawbw@gmail.com
Alfeus Risky Defika fika.veus@gmail.com
Mochammad Shobirin Shobirinmuhammad06@gmail.com
Intan Nilatus S. Intan8nila@gmail.com Crowd funding and Designer for T-Shirt and
Wolfgang Nellen nellen@uni-kassel.de Coordinator

 

We especially thank the Central Laboratory at Brawijaya University for providing lab space and material.

 

Experiment

Hot aqueous extracts have been prepared from Piper betle and the other herbs .

A weak growth inhibition was observed on bacterial lawns. We try to improve that by a) concentrating the extract, b) using cold aqueous extraction, c) using ethanol for extraction.

 

Teaching material

A basic PowerPoint presentation has been prepared. This will be improved and extended within the next weeks. The working protocol is still being developed.

 

Anders Award

The application for the Anders Award has been written and submitted to the German Genetics Society.